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  • Title: Analysing overexpression of L-valine biosynthesis genes in pyruvate-dehydrogenase-deficient Corynebacterium glutamicum.
    Author: Bartek T, Zönnchen E, Klein B, Gerstmeir R, Makus P, Lang S, Oldiges M.
    Journal: J Ind Microbiol Biotechnol; 2010 Mar; 37(3):263-70. PubMed ID: 20012552.
    Abstract:
    L-valine biosynthesis was analysed by comparing different plasmids in pyruvate-dehydrogenase-deficient Corynebacterium glutamicum strains in order to achieve an optimal production strain. The plasmids contained different combinations of the genes ilvBNCDE encoding for the L-valine forming pathway. It was shown that overexpression of the ilvBN genes encoding acetolactate synthase is obligatory for efficient pyruvate conversion and to prevent L-alanine as a by-product. In contrast to earlier studies, overexpression of ilvE encoding transaminase B is favourable in pyruvate-dehydrogenase-negative strains. Its amplification enhanced L-valine formation and avoided extra- and intracellular accumulation of ketoisovalerate.
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