These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Quantitative detection of NADH by in vitro bacterial luciferase bioluminescent].
    Author: Mei C, Wang J, Lin H, Wang J.
    Journal: Wei Sheng Wu Xue Bao; 2009 Sep; 49(9):1223-8. PubMed ID: 20030062.
    Abstract:
    OBJECTIVE: The study aimed at establishing the bacterial luciferase: FMN-NADH oxidoreductase bioluminescent system in vitro and evaluating its potential for quantitative detection of NADH. METHODS: By optimizing the amount of substrates, we set up the coupled luciferase: FMN-NADH oxidoreductase bioluminescent system in vitro, based on the crude extract from Photobacterium leiognathi YL. RESULTS: The in vitro coupled bacterial luciferase: FMN-NADH oxidoreductase bioluminescent system was: 1 mL crude extract, 27 mmol/L Dodecane 100 microL, 10 mmol/L FMN-Na 0.5 microL and 0.14 mmol/L NADH 300 microL. Furthermore, we developed a method for quantitative detection of NADH according to the bioluminescence of NADH catalyzed of bacterial luciferase: FMN-NADH oxidoreductase system in vitro. A good linear relationship of NADH concentration was in a range of 1.0 x 10(-10) to 1.0 x 10(-8) mol/L. CONCLUSION: The bacterial luciferase: FMN-NADH oxidoreductase system used to measure NADH concentration was a good attempt to detect living bacterial cells in the fields of environment, food sanitation and other related.
    [Abstract] [Full Text] [Related] [New Search]