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  • Title: The effect of phytohormones on safflower regeneration plant.
    Author: Yang J, Xiong LD, Li TH, Li HY, Qu Q, Fu HQ, Liu XM, Li XK.
    Journal: Zhong Yao Cai; 2009 Sep; 32(9):1335-8. PubMed ID: 20034206.
    Abstract:
    OBJECTIVE: To study the effect of phytohormones on the regeneration of safflower, in order to provide a foundation for the rapid propagation of safflower seeds and genetic transformation. METHODS: The experiment used the cotyledons as the explant and studied the influence of calli induction, differentiation and rooting by phytohormones. RESULTS: 1. Different hormones by single using induced calli, but didn't directly produce adventitious buds; 2. With the combination of different phytohormones on the differentiation of adventitious buds were significantly different, the roots were also significantly different; 3. Cotyledon calli induction medium: MS + 3% Sucrose + 0.7% Agar + 1.0 mg/L NAA; differentiation medium: MS + 3% Sucrose + 0.7% Agar + 0.2 mg/L NAA + 1.0 mg/L 6-BA; rooting medium: 1/4 MS + 2.0 mg/L NAA + 0.5 mg/L IAA. CONCLUSION: We have screened out the best culture medium of calli induction, differentiation and rooting via using singularity phytohormone and two phytohormones.
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