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  • Title: [Identification and genotyping of oncogenic type of human papillomavirus in paraffin-embedded cervical cancer samples in Guangzhou].
    Author: Zeng L, Yu SY, Gou SQ, Yun JP, Zhang J.
    Journal: Nan Fang Yi Ke Da Xue Xue Bao; 2009 Dec; 29(12):2485-7. PubMed ID: 20034908.
    Abstract:
    OBJECTIVE: To investigate human papillomavirus (HPV) infection and genotyping in patients with cervical cancer in Guangzhou in the last 3 decades. METHODS: HPV L1 gene fragment in paraffin-embedded cervical cancer samples was amplified by HPV-specific PCR with consensus primers, and typing of HPV strains was performed on the basis of sequence analysis of the PCR products. RESULTS: The positivity rates of HPV DNA was 26.2% in the 99 cases of cervical cancer. Five HPV genotypes were identified including HPV16, 18, 33, 52 and 58. CONCLUSION: HPV16, 58 and 33 are the most common genotypes of HPV, accounting for over 88.4% in the total infected cases, suggesting that the HPV genotypes closely related to cervical cancer is more centralized in Guangzhou.
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