These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Foreign anions modulate volume set point of sheep erythrocyte K-Cl cotransport.
    Author: Lauf PK.
    Journal: Am J Physiol; 1991 Mar; 260(3 Pt 1):C503-12. PubMed ID: 2003576.
    Abstract:
    Preequilibration at 37 degrees C in isosmotic media with Cl replaced by lyotropic (foreign) anions reversibly increased Cl-dependent K efflux and Rb influx, the inhibition by furosemide, and thus K-Cl cotransport in low-K but not in high-K sheep erythrocytes with the following order of effectiveness: SCN greater than I greater than NO3 greater than Cl = Br. This effect depended on time, temperature, and anion concentration and was reversible. Preincubation in isosmotic SCN at 37 degrees C stimulated K-Cl flux in anisosmotic Cl media (370-240 mosM) by increasing the volume sensitivity through shifting the point of zero K-Cl flux by approximately 100 mosmol. Thus even shrunken cells exhibited K-Cl cotransport. Preincubation in hyperosmotic SCN or Cl (440 mosM) followed by K flux in hyposmotic Cl (240 mosM) caused a 30-min lag phase that was absent when cells were swollen only. Hence, foreign anions increased the K flux rate in Cl, suggesting upregulation of K-Cl cotransport through new sites or higher turnover per transporter. The anions must act directly on proteins and/or lipids as the accompanying intracellular pH (pHi) changes were too small to attribute the K-Cl flux activation to cellular acidification. After thiol alkylation, which also activates K-Cl cotransport, SCN preexposure at 37 degrees C became ineffective. Carbethoxylation significantly reduced the foreign anion enhancement of K-Cl cotransport and abolished K efflux in Br. It is concluded that interaction of anions through carbethoxylation-sensitive sites with thiols may determine the level of K-Cl cotransport activity.
    [Abstract] [Full Text] [Related] [New Search]