These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Tunable fractionation of nucleic acids.
    Author: Salimullah M, Kato S, Murata M, Kawazu C, Plessy C, Carninci P.
    Journal: Biotechniques; 2009 Dec; 47(6):1041-3. PubMed ID: 20041857.
    Abstract:
    We developed a method for selective purification of DNA using the cationic detergent, cetyltrimethylammonium bromide (CTAB), accompanied with urea and controlled high-salt (NaCl) concentration. This method is effective for rapid separation of DNA fragments from artifacts such as PCR primer dimers or ligation adapters. The CTAB-associated purification completely removed the short PCR artifacts and primers, as well as enzymes and buffer, while recovering a sufficient quantity of amplicons for subsequent experiments such as preparation of libraries. This method could also be applied to the fractionation of nucleic acids generated by other types of reactions.
    [Abstract] [Full Text] [Related] [New Search]