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  • Title: Simultaneous determination of quinolones for veterinary use by high-performance liquid chromatography with electrochemical detection.
    Author: Rodríguez Cáceres MI, Guiberteau Cabanillas A, Galeano Díaz T, Martínez Cañas MA.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2010 Feb 01; 878(3-4):398-402. PubMed ID: 20042380.
    Abstract:
    A selective method based on high-performance liquid chromatography with electrochemical detection (HPLC-ECD) has been developed to enable simultaneous determination of three fluoroquinolones (FQs), namely danofloxacin (DANO), difloxacin (DIFLO) and sarafloxacin (SARA). The fluoroquinolones are separated on a Novapack C-18 column and detected in a high sensitivity amperometric cell at a potential of +0.8 V. Solid-phase extraction was used for the extraction of the analytes in real samples. The range of concentration examined varied from 10 to 150 ng g(-1) for danofloxacin, from 25 to 100 ng g(-1) for sarafloxacin and from 50 to 315 ng g(-1) for difloxacin, respectively. The method presents detection limits under 10 ng g(-1) and recoveries around 90% for the three analytes have been obtained in the experiments with fortified samples. This HPLC-ECD approach can be useful in the routine analysis of antibacterial residues being less expensive and less complicated than other more powerful tools as hyphenated techniques.
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