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  • Title: Label-free femtomolar detection of target DNA by impedimetric DNA sensor based on poly(pyrrole-nitrilotriacetic acid) film.
    Author: Baur J, Gondran C, Holzinger M, Defrancq E, Perrot H, Cosnier S.
    Journal: Anal Chem; 2010 Feb 01; 82(3):1066-72. PubMed ID: 20043643.
    Abstract:
    An ultrahigh performance impedimetric DNA sensor is presented showing detection limits in the femtomolar range. This electrochemical setup was constructed initially by electrogeneration of poly(11-pyrrol-1-yl-undecanoic acid N(alpha'),N(alpha)-bis(carboxymethyl)-L-lysine amide) (poly(pyrrole-NTA)) film. The latter was then modified by the coordination of Cu(2+) ions onto the chelating NTA centers followed by the immobilization of the ssHIV-DNA previously modified by a polyhistidine tag by affinity binding. The immobilization of the DNA probe and hybridization with the complementary target ssHIV-DNA were investigated using fluorescence microscopy and quantified with quartz crystal microbalance experiments leading to DNA probe and duplex coverage of 1.7 x 10(-11) and 7.7 x 10(-12) mol cm(-2), respectively. The duplex formation was corroborated by amperometric measurements through the duplex labeling by a glucose oxidase. In the presence of hydroquinone as redox indicator, the DNA sensor was applied to the impedimetric detection of target DNA without a labeling step. A linear quantification of the HIV DNA target was carried out in the range 10(-15) to 10(-8) mol L(-1).
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