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  • Title: Transformation of the filamentous fungus Gibberella fujikuroi using the Aspergillus niger niaD gene encoding nitrate reductase.
    Author: Sànchez-Fernàndez R, Unkles SE, Campbell EI, Macro JA, Cerdà-Olmedo E, Kinghorn JR.
    Journal: Mol Gen Genet; 1991 Feb; 225(2):231-3. PubMed ID: 2005864.
    Abstract:
    A transformation system for Gibberella fujikuroi based on the Aspergillus niger nitrate reductase gene (niaD) was developed. A strain (designated SG140) carrying a non-reverting niaD mutation (niaD11) was generated by screening mutagenised cells for non-growth on nitrate as sole nitrogen source. Transformation frequencies of 1-2 transformants per microgram DNA were observed when strain SG140 was transformed to nitrate utilisation. Southern blot analyses of niaD+ transformants showed that the vector DNA sequences were integrated into the chromosomal DNA. The results demonstrate that the A. niger niaD gene is expressed in G. fujikuroi.
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