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  • Title: [The investigation of dynamic changes of erythropoietin content of the erythroblast island cultures].
    Author: Sheviakov SA, Zakharov IuM.
    Journal: Ross Fiziol Zh Im I M Sechenova; 2009 Nov; 95(11):1207-15. PubMed ID: 20058818.
    Abstract:
    Dynamic changes of erythropoietin content on the 6th, 12th, 24 and 48th hours of cultivation in bone marrow erythropoietic island liquid short-term cultures were investigated. The initial doses of 0.25, 0.5, 1 mE/ml oferythropoietin was used, and one series with no erythropoietin as control. In culture with 0.25 mE/ml of erythropoietin, its reduction was registered from the 6th to 48th hours of cultivation with relatively lower number of erythroblast islands in state of involution, and a higher number in the state of reconstruction. In cultures with 0.5 mE/ml oferythropoietin, the same erythropoietin reduction was registered with relatively higher numbers of erythroblast islands in the state of reconstruction, and with an unexpected elevation of erythropoietin concentration on the 48th hour of cultivation. In cultures with 1 mE/ml oferythropoietin, such unexpected rise in erythropoietin content was registered in 24 hours, accompanied by relatively higher number of erythroblast islands in the state of reconstruction.
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