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  • Title: Encephalomyocarditis virus infection of captive elephants.
    Author: Simpson CF, Lewis AL, Gaskin JM.
    Journal: J Am Vet Med Assoc; 1977 Nov 01; 171(9):902-5. PubMed ID: 200596.
    Abstract:
    Four African elephants at each of 2 widely separated zoologic gardens in Florida died following a fulminating illness. Tissue suspensions obtained from an elephant from each of the zoologic gardens were inoculated into newborn mice, 3- to 4-week-old mice, and buffalo green monkey and baby hamster kidney cell cultures. Encephalitis and myocarditis developed in the mice. The cell cultures were destroyed within 24 to 72 hours, and intracytoplasmic viral inclusions were observed in infected cells by electron microscopy. The viral agent was neutralized by known antiserum to encephalomyocarditis virus.
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