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  • Title: A new assignment technique of 2D-NMR spectra by spin-lock sequence to a tripeptide containing tryptophan in water.
    Author: Watanabe E, Yamakura F, Kan H.
    Journal: Magn Reson Chem; 2010 Mar; 48(3):205-9. PubMed ID: 20063348.
    Abstract:
    We developed a new assignment technique of tryptophan residues using pulsed field gradient TOCSY-ROESY (PFG-TORO) and pulsed field gradient TOCSY-ROESY-TOCSY (PFG-TOROTO) techniques in water. Connectivity from betaH to zeta2H (H-7) via epsilon1H (H-1) and delta1H (H-2) in the TORO spectrum and from betaH to zeta3H (H-5) and eta2H (H-6) via epsilon1H (H-1) and delta1H (H-2) in the TOROTO spectrum could be able to assign each of the protons of the indole rings.
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