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  • Title: Quantitation of opioids in blood and urine using gas chromatography-mass spectrometry (GC-MS).
    Author: Goldberger BA, Chronister CW, Merves ML.
    Journal: Methods Mol Biol; 2010; 603():399-410. PubMed ID: 20077092.
    Abstract:
    The opioid and 6-acetylmorphine assays utilize gas chromatography-mass spectrometry (GC-MS) for the analysis of morphine, codeine, hydromorphone, hydrocodone, and 6-acetylmorphine in blood and urine. The specimens are fortified with deuterated internal standard and a five-point calibration curve is constructed. Specimens are extracted by mixed-mode solid phase extraction. The morphine, codeine, hydromorphone, hydrocodone, and 6-acetylmorphine extracts are derivatized with N-methyl-bis(trifluoroacetamide) (MBTFA) producing trifluoroacetyl derivatives. The final extracts are then analyzed using selected ion monitoring GC-MS.
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