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Title: [Genetype characteristics of enterovirus type 71 in Shenzhen from 2005 to 2008]. Author: He YQ, Qi R, Yang H, Xian HX, Yao XJ, Yang F, Zhang HL, Yang XK, Xu WB. Journal: Bing Du Xue Bao; 2009 Nov; 25(6):415-9. PubMed ID: 20077930. Abstract: Genetic characteristics of enterovirus type 71 in Shenzhen from 2005 to 2008 were analyzed. All samples were detected by RT-PCR using EV71-specific primers. The VP1s of EV71 strains were amplified and sequenced. A phylogenetic tree was constructed by comparison of the sequences with those of subgenotype A, B and C using DNASTAR, BioEdit and Mega 3.1 softwares. The VP1 nucleotide sequences of 17 strains of Shenzhen shared 95.3%-99.4% identities with cluster C4a, and one strain shared 93.0%-95.6% identities with cluster C4b. The homogeneity of the VP1 nucleotide sequence with subgenotype A and B was 81.8%-86.0%. Among 18 strains, the homogeneity of the VP1 nucleotide sequence was between 92.5%-100%. All EV71 strains circulating in Shenzhen were of subgenotype C4. The predominant strain of EV71 belonged to cluster C4a, also there was cluster C4b.[Abstract] [Full Text] [Related] [New Search]