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  • Title: UBE2S drives elongation of K11-linked ubiquitin chains by the anaphase-promoting complex.
    Author: Wu T, Merbl Y, Huo Y, Gallop JL, Tzur A, Kirschner MW.
    Journal: Proc Natl Acad Sci U S A; 2010 Jan 26; 107(4):1355-60. PubMed ID: 20080579.
    Abstract:
    The Anaphase-Promoting Complex (APC) is an E3 ubiquitin ligase that regulates mitosis and G1 by sequentially targeting cell-cycle regulators for ubiquitination and proteasomal degradation. The mechanism of ubiquitin chain formation by APC and the resultant chain topology remains controversial. By using a single-lysine APC substrate to dissect the topology of ubiquitinated substrates, we find that APC-catalyzed ubiquitination has an intrinsic preference for the K11 linkage of ubiquitin that is essential for substrate degradation. K11 specificity is determined by an E2 enzyme, UBE2S/E2-EPF, that elongates ubiquitin chains after the substrates are pre-ubiquitinated by UbcH10 or UbcH5. UBE2S copurifies with APC; dominant-negative Ube2S slows down APC substrate degradation in functional cell-cycle extracts. We propose that Ube2S is a critical, unique component of the APC ubiquitination pathway.
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