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  • Title: In vitro propagation of rose.
    Author: Pati PK, Kaur N, Sharma M, Ahuja PS.
    Journal: Methods Mol Biol; 2010; 589():163-76. PubMed ID: 20099100.
    Abstract:
    In vitro propagation of rose is an important tool for rapid multiplication and development of new cultivars with desirable traits. However, successful in vitro propagation requires an understanding of specific requirements and precise manipulation of various factors. Efficient protocols for different stages of micropropagation using apical buds or nodal segments are currently available. Recently, new challenges for refinements of protocols for high rate of shoot multiplication and development of cost effective methods has gained importance. Significance of the liquid static culture for shoot proliferation and root induction for rose has also gained prominence. Other distinct approaches of in vitro propagation include organogenesis and embryogenesis. These approaches are important for the successful implementation of various biotechnological techniques used for rose improvement programmes. Types of explants, media and optimization of conditions are major factors for successful regeneration of rose.
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