These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Enantiomeric separation of S-zopiclone and its R-enantiomer in bulk drug samples by validated chiral RP-HPLC.
    Author: Sangaraju S, Lakshmi Kanth M, Rao BM, Someswararao N.
    Journal: Pharmazie; 2009 Nov; 64(11):717-9. PubMed ID: 20099514.
    Abstract:
    A simple and selective isocratic chiral RP-HPLC method was developed for the enantiomeric purity determination of S-zopiclone and the quantitative determination of R-zopiclone in bulk drug samples. Enantiomeric separation was achieved using Chiralcel OD-RH 150 x 4.6 mm, 5 microm particle size column at 25 degrees C using a mobile phase of 10 mM ammonium acetate and acetonitrile in ratio of 60:40 (v/v) as mobile phase at a flow rate of 1.0 mL x min(-1) and UV detection at 306 nm. The method resolves the R-zopiclone and S-zopiclone with resolution (Rs) greater than 1.6. The limit of detection (LOD) and limit of quantitation (LOQ) of the R-enantiomer were 0.12 microg x mL(-1) and 0.40 microg x mL(-1) respectively, for 10 microL injection volume. The percentage RSD of the peak area of six replicate injections of R-zopiclone at LOQ concentration was 4.6. The percentage recoveries of R-enantiomer from S-zopiclone were ranged from 97.3 to 99.8. Developed method was found to be selective in presence potential impurities. The developed chiral RP-HPLC method was validated with respect to precision, linearity, accuracy, robustness and ruggedness. The test solution and mobile phase was found to be stable up to 24 h after preparation.
    [Abstract] [Full Text] [Related] [New Search]