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  • Title: Nanofibrous membrane based tyrosinase-biosensor for the detection of phenolic compounds.
    Author: Arecchi A, Scampicchio M, Drusch S, Mannino S.
    Journal: Anal Chim Acta; 2010 Feb 05; 659(1-2):133-6. PubMed ID: 20103115.
    Abstract:
    A tyrosinase-modified electrode is described to be used as amperometric biosensor for the detection of phenolic compounds in food. The enzyme has been immobilized by drop-coating on a glassy carbon electrode covered by a polyamidic nanofibrous membrane prepared by electrospinning. With respect to others, the selectivity of the designed tyrosinase-biosensor resulted modified by the presence of the nanostructured coating which seems to affect the permeability of phenols as a function of the pH of the solution and of their dissociation constants. The biosensor exhibits a response time of 16 s, a detection limit of 0.05 microM, and a linearity up to 100 microM (slope: -304 nA microM(-1); intercept: -191 nA, r(2)=0.996, n=19). Among others, it can be successfully used for monitoring in real time the release kinetics of phenols encapsulated in polymeric microcapsules.
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