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  • Title: [RNA-synthesizing complex of the Sendai virus in the nucleoli of infected Erlich ascitic carcinoma cells].
    Author: Agafonova LV, Vladimirtseva EA, Bukrinskaia AG.
    Journal: Vopr Virusol; 1977; (5):544-51. PubMed ID: 201099.
    Abstract:
    A virus-specific complex with sedimentation constant of 250S and buoyant density 1.35 g/cm3 in cesium chloride density gradient is formed within 20--24 hours after infection in nucleoli of Ehrlich ascitic carcinoma cells infected with Sendai virus. The complex contains a rapidly sedimenting RNA (70--90S) which is about 50% stable to RN-ase. The complex has RNA-polymerase activity in a cell-free system; the product of the RNA-polymerase reaction is a RNA with sedimentation constant 50S. It is suggested that the virus-specific structure with sedimentation constant 250 S found in nucleoli is the Sendai virus replicative complex.
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