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  • Title: [Identify the original plant of Forsythia suspensa by nucleotide sequence].
    Author: Yuan WJ, Xu SM, Zhang WR.
    Journal: Zhong Yao Cai; 2009 Oct; 32(10):1524-6. PubMed ID: 20112711.
    Abstract:
    OBJECTIVE: To investigate the means of distinguishing the original plant of Forsythia suspensa from confusion. METHODS: To amplify the chloroplast matK gene by PCR using a consensus primer set and determine their nucleotide sequence by PCR direct sequencing. The ITS sequences were gained from NCBI. The characteristic analysis of matK and ITS sequences were generated using Clustal aligned. RESULTS: There were 30 bp and 8 bp unique nucleotide in ITS and matK sequence in Forsythia suspensa. The matK gene and ITS sequences might be good molecular marker to be used to identify the original plant of Forsythia suspensa. CONCLUSION: The sequence analysis of matK gene ITS sequences might become the mean to identify the original plant of Forsythia suspensa.
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