These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Effect of Cordyceps Cinensis extractant on apoptosis and expression of Toll-like receptor 4 mRNA in the ischemia-reperfusion injured NRK-52E cells].
    Author: Zhou Q, Hu S.
    Journal: Zhong Nan Da Xue Xue Bao Yi Xue Ban; 2010 Jan; 35(1):77-84. PubMed ID: 20130368.
    Abstract:
    OBJECTIVE: To observe the apoptosis and expression of Toll-like receptor (TLR)-4's mRNA in ischemia-reperfusion injured rat renal tubular epithelia cells (NRK-52E) in vitro, and effect of Cordyceps Sinensis (CS) extractant. METHODS: Cultured NRK-52E cells were divided into a control group, a model group, and a CS preincubated group. All first removed media and incubated with antimycin A for 1 h, and then recovered the media to simulate the ischemia-reperfusion injury in vitro. We detected the apoptosis ratio of cells by flow cytometer and the mRNA expression of TLR-4, Bax, Bcl-2 gene by reverse transcription-polymerase chain reaction at different time points. RESULTS: In ischemia-reperfusion injured NRK-52E, the apoptosis ratio rose as time passed (P<0.05). We also observed increased mRNA expression of TLR-4, Bax (P<0.05) and deceased expression of Bcl-2 (P<0.05). Compared with the model group, the CS preincubated NRK-52E cells showed apparent tolerance to ischemia-reperfusion injury, which manifestated lower apoptosis ratio (P<0.05), decreased expression of mRNA of TLR-4, Bax and increased expression of Bcl-2 (All Ps<0.05). CONCLUSION: The number of apoptosis cells and the expression of TLR-4 mRNA increased with ischemia-reperfusion injury of NRK-52E in vitro. CS can prevent the NRK-52E cells from ischemia-reperfusion injury by downregulating TLR-4 gene.
    [Abstract] [Full Text] [Related] [New Search]