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  • Title: Measurement of alkaline phosphatase of intestinal origin in plasma by p-bromotetramisole inhibition.
    Author: Kuwana T, Rosalki SB.
    Journal: J Clin Pathol; 1991 Mar; 44(3):236-7. PubMed ID: 2013625.
    Abstract:
    L-p-bromotetramisole was used to inhibit non-intestinal alkaline phosphatase (of liver or bone origin) (EC 3.1.3.1; ALP) in plasma, and intestinal ALP was measured from the uninhibited activity. The method of determination is convenient and correlated well with measurement by immunocapture assay. If carried out in parallel with wheat-germ lectin precipitation of bone ALP, subtraction of intestinal ALP activity from that of non-bone ALP in the supernatant can be used to measure the ALP that originates from the liver in men and non-pregnant women.
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