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  • Title: Live-cell one- and two-photon uncaging of a far-red emitting acridinone fluorophore.
    Author: Warther D, Bolze F, Léonard J, Gug S, Specht A, Puliti D, Sun XH, Kessler P, Lutz Y, Vonesch JL, Winsor B, Nicoud JF, Goeldner M.
    Journal: J Am Chem Soc; 2010 Mar 03; 132(8):2585-90. PubMed ID: 20141131.
    Abstract:
    Total synthesis and photophysical properties of PENB-DDAO, a photoactivatable 1,3-dichloro-9,9-dimethyl-9H-acridin-2(7)-one (DDAO) derivative of a far-red emitting fluorophore, are described. The photoremovable group of the DDAO phenolic function comprises a donor/acceptor biphenyl platform which allows an efficient (> or = 95%) and rapid (< 15 micros time-range) release of the fluorescent signal and displays remarkable two-photon uncaging cross sections (delta(a) x Phi(u) = 3.7 GM at 740 nm). PENB-DDAO is cell permeable as demonstrated by the triggering of cytoplasmic red fluorescent signal in HeLa cells after one-photon irradiation (lambda(exc) around 360 nm) or by the generation of a red fluorescent signal in a delineated area of a single cell after two-photon photoactivation (lambda(exc) = 770 nm).
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