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  • Title: Microchip electrophoresis for monitoring covalent attachment of poly(ethylene glycol) to proteins.
    Author: Park EJ, Na DH.
    Journal: Anal Biochem; 2010 May 15; 400(2):304-6. PubMed ID: 20149779.
    Abstract:
    A microchip electrophoretic method was applied to monitor and characterize the covalent attachment of poly(ethylene glycol) (PEGylation) of two proteins, alpha-lactalbumin and bovine serum albumin, using several poly(ethylene glycol) (PEG) derivatives with molecular weights from 1 to 20 kDa. This method effectively separated multi-PEGylated proteins in a size-based manner and allowed monitoring of the PEGylation pattern with the advantages of high speed, minimal sample consumption, and high reproducibility. Microchip electrophoresis would be a very useful tool for protein PEGylation studies such as reaction monitoring, purity checks, and characterization of PEGylated protein products.
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