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  • Title: Immobilization of glucose oxidase on Fe3O4/SiO2 magnetic nanoparticles.
    Author: Huang J, Zhao R, Wang H, Zhao W, Ding L.
    Journal: Biotechnol Lett; 2010 Jun; 32(6):817-21. PubMed ID: 20155486.
    Abstract:
    Glucose oxidase (GOD) was covalently immobilized onto Fe(3)O(4)/SiO(2) magnetic nanoparticles (FSMNs) using glutaraldehyde (GA). Optimal immobilization was at pH 6 with 3-aminopropyltriethoxysilane at 2% (v/v), GA at 3% (v/v) and 0.143 g GOD per g carrier. The activity of immobilized GOD was 4,570 U/g at pH 7 and 50 degrees C. The immobilized GOD retained 80% of its initial activity after 6 h at 45 degrees C while free enzyme retained only 20% activity. The immobilized GOD maintained 60% of its initial activity after 6 cycles of repeated use and retained 75% of its initial activity after 1 month at 4 degrees C whereas free enzymes retained 62% of its activity.
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