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  • Title: Purification of beta-toxin from Clostridium perfringens type C.
    Author: Sakurai J, Duncan CL.
    Journal: Infect Immun; 1977 Dec; 18(3):741-5. PubMed ID: 201565.
    Abstract:
    Beta-toxin was purified about 340-fold from culture supernatant fluid of Clostridium perfringens type C with a yield of about 24% in terms of biologically active beta-toxin. The purification involved ammonium sulfate fractionation, gel filtration through Sephadex G-100, isoelectrofocusing in a pH 3 to 6 gradient, and immunoaffinity chromatography. The purified beta-toxin gave a single band on polyacrylamide gel electrophoresis.
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