These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Evaluation of lens epithelial cell differentiation by quantitation of MP26 mRNA relative to gamma-crystallin mRNA in initiation of galactose cataracts in the rat.
    Author: Wen Y, Unakar NJ, Bekhor I.
    Journal: Exp Eye Res; 1991 Mar; 52(3):321-7. PubMed ID: 2015861.
    Abstract:
    It is well established that in response to feeding of galactose to four-week-old rats cataracts develop (Unakar, Genyea, Reddan & Reddy, Exp. Eye Res. 26, 123-33, 1978). Initiation of cataracts leads to epithelial cell proliferation. In the lens, the main intrinsic membrane protein, MP26, is recognized as a fiber cell-specific gene product, and gamma-crystallin is found in terminally differentiated fiber cells. By Northern blot analysis we quantitated the MP26 and gamma-crystallin mRNAs found in the lens at various times spanning from 0 to 96 hr on galactose. We find that by 24 hr, MP26 mRNA, as well as gamma-crystallin mRNA, increased significantly above the zero time levels. The data was also confirmed by in situ hybridization of various lens sections to both gamma-crystallin and MP26 35S-labeled antisense RNA probes. As expected, at later periods beyond 24 hr, the levels of gamma-crystallin and MP26 mRNAs dropped to below the control levels. The initial increase in MP26 and gamma-crystallin mRNAs lead us to conclude that initiation of galactose cataracts appears to support, although for a short time period, both epithelial cell elongation and fiber cell differentiation.
    [Abstract] [Full Text] [Related] [New Search]