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  • Title: Livin gene plays a role in drug resistance of colon cancer cells.
    Author: Wang X, Xu J, Ju S, Ni H, Zhu J, Wang H.
    Journal: Clin Biochem; 2010 May; 43(7-8):655-60. PubMed ID: 20171199.
    Abstract:
    OBJECTIVE: The objective of this study was to investigate the effect of knockdown of Livin expression on reversing drug resistance phenotype of colon cancer HCT-8/V cells. DESIGN AND METHODS: Specific short hairpin RNA (shRNA) was chosen and transfected in human colon cancer HCT-8/V cell line. Cell apoptosis and chemosensitivity were evaluated following downregulation of Livin expression. RESULTS: In the current study, Livin was found to be highly expressed in the HCT-8/V colon cancer cells, which were resistant to several anti-tumor drugs. Knocking down of Livin expression in HCT-8/V cells by specific RNAi facilitated the apoptosis of HCT-8/V cells in response to vincristine (VCR), etoposide (VP-16), and 5-flourouracil (5-FU). Chemosensitivity assay confirmed the results and demonstrated the reversal of drug resistance phenotype of HCT-8/V cells. CONCLUSION: These data suggest that specific silencing of Livin gene expression could be a promising target for further research in clinical chemotherapy of colon cancer.
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