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  • Title: Quantification of intracellular free sodium ions by using a new fluorescent indicator, sodium-binding benzofuran isophthalate in guinea pig myocytes.
    Author: Satoh H, Hayashi H, Noda N, Terada H, Kobayashi A, Yamashita Y, Kawai T, Hirano M, Yamazaki N.
    Journal: Biochem Biophys Res Commun; 1991 Mar 15; 175(2):611-6. PubMed ID: 2018505.
    Abstract:
    Isolated guinea pig myocytes were loaded with the Na(+)-sensitive fluorescent probe, sodium-binding benzofuran isophthalate (SBFI). The 340/380 nm fluorescence ratios were measured with fluorescence microscopy. The distribution of intracellular Na+ concentration ([Na+]i) was homogenous, and the mean resting [Na+]i was 8.4 +/- 0.5 mM. There was a significant relationship (r = 0.66, p less than 0.001) between elevation of [Na+]i and shortening of longitudinal length of the cells, during the perfusion of 100 microM strophanthidin. It is concluded that this method is suitable for measuring [Na+]i in isolated myocytes.
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