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  • Title: Microhardness of dentin underneath fluoride-releasing adhesive systems subjected to cariogenic challenge and fluoride therapy.
    Author: Kirsten GA, Takahashi MK, Rached RN, Giannini M, Souza EM.
    Journal: J Dent; 2010 Jun; 38(6):460-8. PubMed ID: 20193726.
    Abstract:
    OBJECTIVES: The aim of this study was to evaluate the microhardness of dentin subjacent to the bonding interface of composite restorations using fluoride-releasing adhesive systems submitted to pH-cycling regimen associated or not to fluoride therapies. METHODS: Thirty human third molars were prepared with class V cavities with dentin cervical margins. The adhesive systems One-Up Bond F Plus (OU), Prime&Bond NT (NT), Clearfil Protect Bond (CF), Optibond Solo Plus (OP) and also the controls [-] Single Bond 2 (SB) and [+] Ketac Molar (KM) were used previously to composite resin restorations. The restorations were sectioned into four slabs and submitted to different storage media for 15 days: distilled water, pH-cycling, pH-cycling associated to NaF 0.05% and associated to NaF 1.23%. The Knoop microhardness test was performed in dentin at 50, 100, 150 and 300 microm from the adhesive interface. Data was analyzed by three-way ANOVA and Tukey HSD test (p<0.05). RESULTS: KM resulted in significantly higher microhardness when compared to all the adhesive systems at 50 microm, with the exception of OU, that was similar to KM when submitted to pH-cycling alone or associated to 1.23% NaF. Microhardness of dentin was significantly higher with all the tested materials, when pH-cycling was associated to NaF 0.05%, at 50 microm and 100 microm depths. OU resulted in similar dentin hardness at all depths and storage media. CONCLUSIONS: The incorporation of NaF 0.05% fluoride therapy to the cariogenic challenge was capable to recover the original microhardness of dentin at 50 and 100 microm with all the tested materials.
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