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  • Title: Imaging single molecular motor motility with total internal reflection fluorescence microscopy (TIRFM).
    Author: Reck-Peterson SL, Derr ND, Stuurman N.
    Journal: Cold Spring Harb Protoc; 2010 Mar; 2010(3):pdb.prot5399. PubMed ID: 20194468.
    Abstract:
    Total internal reflection fluorescence microscopy (TIRFM) allows fluorescent molecules on or near the plasma membrane to be visualized with a very high signal-to-noise ratio. This strategy has been very successful for imaging molecular machines as they move and do work. We provide here a general protocol for imaging single molecular motors as they move along microtubule tracks. Our protocol is designed for the study of cytoplasmic dynein purified from Saccharomyces cerevisiae, but it represents a general framework for any in vitro single-molecule assay.
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