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  • Title: L-Arginine reduces thioflavin T fluorescence but not fibrillation of bovine serum albumin.
    Author: Liu KN, Wang HY, Chen CY, Wang SS.
    Journal: Amino Acids; 2010 Aug; 39(3):821-9. PubMed ID: 20204431.
    Abstract:
    This work examines the effects of L-arginine (L-Arg) on the aggregation and amyloid fibrillation of bovine serum albumin (BSA). We demonstrate that L-Arg dose-dependently reduces thioflavin T (ThT) fluorescence of BSA within the L-Arg concentration range used (0-1.4 M). However, as revealed by electron microscopy, size exclusion chromatography, and dynamic light scattering results, L-Arg does not prevent amyloid-like fibril formation by BSA. We conclude that L-Arg competes against ThT for binding sites on BSA amyloid-like fibrils, leading to biased results in ThT fluorescence measurements. Moreover, the use of ThT fluorescence assay to screen for potential inhibitors against amyloid fibrillation can give misleading results.
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