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  • Title: [Determination of norisoboldine in Radix Lindera by RP-HPLC].
    Author: Chen J, Chou G, Yang L, Wang C, Wang Z.
    Journal: Zhongguo Zhong Yao Za Zhi; 2009 Nov; 34(21):2774-6. PubMed ID: 20209913.
    Abstract:
    OBJECTIVE: To develop a RP-HPLC method for quantitative determination of norisoboldine in Radix Linderae and to provide valuable data for quality control of Radix Linderae. METHOD: The separation was performed on a Phenomenex Gemini C18 column (4.6 mm x 250 mm, 5 microm) at 25 degrees C using a gradient elution of mobile phase A (0.5% formic acid, adjusted pH 2.25 with triethylamine) and mobile phase B (acetonitrile). The detection wavelength was 280 nm. RESULT: The calibration curve showed a good linearity (r = 0.999 9) within test ranges of 0.015-1.509 microg. The average recovery was 99.58% with RSD 1.4%. CONCLUSION: The developed method is simple, accurate and reliable with good repeatability. It is suitable for quality evaluation of Radix Linderae.
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