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  • Title: High-throughput screening in two dimensions: binding intensity and off-rate on a peptide microarray.
    Author: Greving MP, Belcher PE, Cox CD, Daniel D, Diehnelt CW, Woodbury NW.
    Journal: Anal Biochem; 2010 Jul 01; 402(1):93-5. PubMed ID: 20211590.
    Abstract:
    We report a high-throughput two-dimensional microarray-based screen, incorporating both target binding intensity and off-rate, which can be used to analyze thousands of compounds in a single binding assay. Relative binding intensities and time-resolved dissociation are measured for labeled tumor necrosis factor alpha (TNF-alpha) bound to a peptide microarray. The time-resolved dissociation is fitted to a one-component exponential decay model, from which relative dissociation rates are determined for all peptides with binding intensities above background. We show that most peptides with the slowest off-rates on the microarray also have the slowest off-rates when measured by surface plasmon resonance (SPR).
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