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  • Title: Selection of bacterial antagonists for the biological control of Botrytis cinerea in apple (Malus domestica) and in comparison with application of thiabendazole.
    Author: Peighami-Ashnaei S, Sharifi-Tehrani A, Ahmadzadeh M, Behboudi K.
    Journal: Commun Agric Appl Biol Sci; 2009; 74(3):739-43. PubMed ID: 20222558.
    Abstract:
    In this study fifteen strains of identified Pseudomonas fluorescens and Bacillus subtilis were investigated for biological control activity against Botrytis cinerea. P. fluorescens P-35 and B. subtilis B-16 showed the most inhibitory zone in dual culture assay against B. cinerea, In vitro. After ten days, P. fluorescens P-5 and B. subtilis B-3 showed the considerable results against B. cinerea on apple fruits and could reduce the grey mould from 100% to less than 35%. After twenty days, P. fluorescens P-5 and 8. subtilis B-16 decreased the disease from 100% to less than 65%. Also, application of thiabendazol at 1500 mg/litre was more effective and could reduce the disease from 100% to 30% and 60%, after 10 and 20 days respectively. Results indicated that there is no significant difference among the treatments (thiabendazol and bacterial strains). So, bacterial strains could not only control the disease but also be a reliable replacement instead of Thiabendazol.
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