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  • Title: Molecular cloning of DNA from a sorted human minichromosome.
    Author: Ferretti L, Raimondi E, Gamberi C, Young BD, De Carli L, Sgaramella V.
    Journal: Gene; 1991 Mar 15; 99(2):229-34. PubMed ID: 2022335.
    Abstract:
    A human supernumerary minichromosome (MC), found in a newborn baby and sorted on a fluorescence-activated cell sorter (FACS-440) has been previously described [Ferretti et al., Cytotechnology 1 (1987) 7-12]. We report here on the construction of a library of EcoRI fragments in the phage lambda gtWES.lambda B', starting from 7.5 ng of MC DNA, and describe the isolation of single-copy DNA clones from the library in a two-step procedure. We employed in situ hybridization to unambiguously select the clones specific for the MC, and used three of these clones to demonstrate that it originated from chromosome 9.
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