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Title: A visible dye-based staining method for DNA in polyacrylamide gels by ethyl violet. Author: Cong WT, Zhu ZX, He HZ, Jin Y, Jiang CX, Choi JK, Jin LT, Li XK. Journal: Anal Biochem; 2010 Jul 01; 402(1):99-101. PubMed ID: 20230772. Abstract: We describe a visible dye-based staining method for DNA in polyacrylamide gels using ethyl violet (EV). The novel method is a background-free, sensitive, economical, and simple procedure involving only staining and washing steps that can be completed within 30 min. As little as 0.8-1.6 ng of phiX174 DNA/HaeIII can be detected by EV, which is about eightfold more sensitive than Nile blue (NB) stain and twofold less sensitive than ethidium bromide (EB) stain.[Abstract] [Full Text] [Related] [New Search]