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  • Title: [X-sNP genotyping using the TaqMan probe technology].
    Author: Zhang SH, Li L, Li CT, Zhao SM.
    Journal: Fa Yi Xue Za Zhi; 2010 Feb; 26(1):22-5. PubMed ID: 20232738.
    Abstract:
    OBJECTIVE: To develop a rapid, accurate and economical real time fluorescence PCR method with TaqMan probe technology to detect the X chromosome single nucleotide polymorphism (X-SNP). METHODS: TaqMan probes and polymerase chain reaction primers were respectively designed according to the 13 X-SNP. Then, the X-SNP were genotyped after the amplification by real time fluorescence PCR. RESULTS: All the loci follow the Hardy-Weinberg equilibrium. The polymorphic information content for 13 distinct loci varied between 0.3497 and 0.3750 while the heterozygosity ranged from 0.4537 to 0.5021. A real time fluorescent PCR method based on TaqMan probe was successfully developed and the results were accordant with those analyzed by DNA sequencing of the 13 X-SNP. CONCLUSION: The allele specific real time fluorescence PCR based on TaqMan probe is a sensitive, simple technology and suitable for rapid analysis of XSNP. All the loci show highly polymorphic and may be potential in forensic genetics.
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