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  • Title: A simple method for concentration of enteroviruses and rotaviruses from cell culture harvests using membrane filters.
    Author: Farrah SR, Goyal SM, Gerba CP, Conklin RH, Wallis C, Melnick JL, DuPont HL.
    Journal: Intervirology; 1978; 9(1):56-9. PubMed ID: 202572.
    Abstract:
    Organic compounds in cell culture harvests known as membrane-coating components (MCC) prevent virus adsorption to membrane filters. Blending cell culture harvests with fluorocarbon removed the MCC and permitted adsorption of virus in acidified harvests to epoxy-fiberglass filters. Subsequent elution with high pH buffer resulted in recovery of greater than 90% of the virus with concentrations of up to 100-fold.
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