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  • Title: Characterization of HIV-1 REV protein: binding stoichiometry and minimal RNA substrate.
    Author: Cook KS, Fisk GJ, Hauber J, Usman N, Daly TJ, Rusche JR.
    Journal: Nucleic Acids Res; 1991 Apr 11; 19(7):1577-83. PubMed ID: 2027765.
    Abstract:
    The HIV-1 REV protein binds to the stem II region of the REV-responsive element (RNA). Studies to further define the RNA sequence and structure specifically bound by REV protein identify a minimal RNA element of 40 nucleotides. Analysis of RNA fragments by gel retardation and filter binding suggest that a core element composed of one particular stem with flanking sequences capable of forming a second double stranded region is essential for specific recognition by REV protein. Stable REV-RNA complexes are formed in a stoichiometry of 1 REV: 1 RNA. The minimal RNA element binds 1 REV molecule while the stem II saturates at 3 REV molecules per RNA. These results establish that REV recognizes a primary binding site within the RRE and support the notion that the initial viral transcript binding event involves a monomeric REV protein.
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