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  • Title: [Molecular epidemiological markers of Legionella pneumophila in the Valencian Community (III). Peptide profiles].
    Author: Muñoz C, Catalán V, Borrás R, García-de-Lomas J.
    Journal: Enferm Infecc Microbiol Clin; 1991 Jan; 9(1):18-21. PubMed ID: 2029553.
    Abstract:
    Electrophoretic profiles using soluble peptides obtained from Legionella pneumophila serogroup 1 have been studied to be used as epidemiological markers. Using 8.5% polyacrylamide gels (SDS-PAGE) a unique profile common to environmental and clinical isolates has been detected showing 2 differential bands: a 150 kD and a 230 kD band present in only one clinical isolate. In order to achieve better resolution in the peptide profile 10 to 20% gradient SDS-PAGE has been used, confirming the existence of a common profile and 2 subtypes as well as another profile present in a smaller number of strains.
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