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  • Title: Cryopreservation of Thymus moroderi by droplet vitrification.
    Author: Marco-Medina A, Luis Casas J, Swennen R, Panis B.
    Journal: Cryo Letters; 2010; 31(1):14-23. PubMed ID: 20309505.
    Abstract:
    Thymus moroderi Pau ex Martinez (Labiatae) was successfully cryopreserved using the droplet vitrification method. After 20 min in loading solution at room temperature, shoot tips were dehydrated with PVS2 at 0 degree C for 30 min and immersed into LN. For thawing, shoot-tips were transferred into recovery solution for 15 min. A test of different recovery media revealed that the best results were obtained when the medium was supplement with 0.275 micromolar BA.
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