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  • Title: A comparative identification of ochratoxin A in longan fruit pulp by high performance liquid chromatography-fluorescence detection and electron spray ionization-mass spectrometry.
    Author: Li J, Xie H, Yang B, Dong X, Feng L, Chen F, Jiang Y.
    Journal: Molecules; 2010 Jan 29; 15(2):680-8. PubMed ID: 20335938.
    Abstract:
    Harvested longan (Dimocarpus longan Lour.) fruit are susceptible to decay caused by both bacterial and fungal infections. Ochratoxin A (OTA) is a kind of mycotoxin produced by a number of fungi. In this study, OTA was extracted from longan fruit pulp by 80% methanol and then loaded on C-18 solid-phase extraction columns. The extract solution was then analyzed by high-performance liquid chromatography - fluorescence detection (HPLC-FD) and an electron spray ionization-mass spectrometry (ESI-MS), respectively. The HPLC-FD analysis showed that a compound similar to OTA might exist in longan fruit pulp, but further analysis by the ESI-MS method demonstrated that OTA was not present in the longan pulp, indicating that the presence of OTA in longan fruit pulp detected by the HPLC-FD analysis needed to be confirmed by the ESI-MS method.
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