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  • Title: Cloning and expression profiles of two isoforms of a CHH-like gene specifically expressed in male Chinese shrimp, Fenneropenaeus chinensis.
    Author: Li S, Li F, Wang B, Xie Y, Wen R, Xiang J.
    Journal: Gen Comp Endocrinol; 2010 Jun 01; 167(2):308-16. PubMed ID: 20347822.
    Abstract:
    Two full-length cDNA sequences (Fc-CHH1, Fc-CHH2) encoding a crustacean hyperglycemic hormone (CHH) precursor homolog and their DNA sequences were cloned from Chinese shrimp Fenneropenaeus chinensis. The deduced amino acid sequences of them are predicted to contain a signal peptide and a mature peptide. The mature peptides of Fc-CHH1 and Fc-CHH2 shared 78% identity, but they showed low identities (less than 40%) to CHH peptides from other species. Both Fc-CHH1 and Fc-CHH2 proteins contain six highly conserved cysteine residues which are characteristic of the CHH family peptides. The transcripts of Fc-CHH1 and Fc-CHH2 were shown to be specifically present in the spermatophore sac of mature male Chinese shrimp through reverse transcription-polymerase chain reaction (RT-PCR) detection. The transcripts of Fc-CHH1 and Fc-CHH2 begin to appear at the immature stage (115 days after the first post-larvae stage) when the spermatophore sac was first observed to be appeared. In situ hybridization analyses showed that Fc-CHH1 and Fc-CHH2 transcripts located at the epithelial cells in the internal wall of the spermatophore sac. In the cloned DNA sequences of Fc-CHH1 and Fc-CHH2, the predicted transcription factor binding sites in the 5' flanking sequences are different from those previously reported for CHH family genes of crustacean. To our knowledge, these are novel CHH-like genes expressed specifically in male shrimp. Their function needs to be further investigated.
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