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  • Title: Influence of media and testing methodology on susceptibility to tigecycline of Enterobacteriaceae with reported high tigecycline MIC.
    Author: Torrico M, González N, Giménez MJ, Alou L, Sevillano D, Navarro D, Díaz-Antolín MP, Larrosa N, Aguilar L, Garcia-Escribano N.
    Journal: J Clin Microbiol; 2010 Jun; 48(6):2243-6. PubMed ID: 20351208.
    Abstract:
    The tigecycline susceptibility of six different Enterobacteriaceae strains with reported high tigecycline MICs was determined in quintuplicate by four methodologies using Mueller-Hinton agar and broth from six manufacturers. The MICs determined by Etest were a >or=1-fold dilution lower than those determined by broth microdilution and agar dilution, with the highest modal values given by agar dilution. The highest modal MICs were obtained using Oxoid medium, and the lowest inhibition zone values (disc diffusion) were obtained using Oxoid and bioMérieux media. The lowest MICs were obtained by Etest using Difco or Merck media.
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