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  • Title: [Determination of 61 central nervous system drugs in plasma by protein precipitation-high performance liquid chromatography].
    Author: Zhang Y, Chen C, Lin L, Chen Y.
    Journal: Se Pu; 2009 Nov; 27(6):787-93. PubMed ID: 20352932.
    Abstract:
    A method was established for the determination of 61 central nervous system drugs in plasma by using protein precipitation combined with high performance liquid chromatography-diode array detection (HPLC-DAD). A volume of 1.5 mL acetonitrile was added into 1 mL plasma, after vortex, centrifugation and filtration, the supernatant was directly injected into HPLC. The separation was performed on an Agilent TC-C18 column (250 mm x 4.6 mm, 5 microm) with acetonitrile and phosphate buffer solution as mobile phase by gradient elution at a flow rate of 1.5 mL/min. The detection wavelength was 210 nm; full spectra were recorded from 200-364 nm. The recoveries of 61 drugs were larger than 80% with the relative standard deviations (RSDs) ranged from 0.94% to 11.23%. The protein precipitation method is simple, rapid, low-cost with good recoveries, reproducibility and suitable for the general pretreatment of the systematic toxicological analysis (STA) of the 61 drugs.
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