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Title: [Enhancing ethanol production using thermophilic yeast by response surface methodology]. Author: Shen N, Wang Q, Lu Y, Qin Y, Huang R. Journal: Sheng Wu Gong Cheng Xue Bao; 2010 Jan; 26(1):42-7. PubMed ID: 20353091. Abstract: We optimized the conditions of simultaneous saccharification and fermentation (SSF) from cassava flour into high-concentration ethanol by thermophilic yeast GXASY-10. Based on the single factor experiment, we screened the important parameters by Plackeet-burman design. We used the path of steepest ascent to approach to the biggest region of ethanol production subsequently. Then, we obtained the optimum values of the parameters by Box-Behnken design. The results showed that the important parameters were the liquefaction time, glucosidase dosages and initial concentration of cassava flour (substrate). The optimum technical conditions were as follows: liquefaction time 35 min, glucosidase dosages 1.21 AGU/g substrate and initial substrate concentration 37.62%. Under such optimum conditions, the ethanol yield of 20 L fermentor reached 16.07% (V/W) after 48 h fermentation at 37 degrees C and 100 r/min. The ethanol content increased 33% than that under the original condition.[Abstract] [Full Text] [Related] [New Search]