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  • Title: Pouring linear gradient gels with a gradient former.
    Author: Simpson RJ.
    Journal: Cold Spring Harb Protoc; 2010 Apr; 2010(4):pdb.prot5411. PubMed ID: 20360365.
    Abstract:
    Linear gradient gels, which are polyacrylamide gels having a gradient of increasing acrylamide concentration (and linearly decreasing pore size), can have advantages over fixed-concentration SDS-PAGE gels. First, a much greater range of protein M(r) values can be separated on a linear gradient gel than on a fixed-concentration gel. Second, there is a greater likelihood of resolving proteins with very similar M(r) values on gradient gels than on fixed-concentration gels. This protocol describes the pouring of linear gradient gels with a gradient former. The most common gradient gel contains 4%-20% acrylamide; however, the range of acrylamide concentrations should be chosen on the basis of the size of the proteins being separated.
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