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  • Title: A new hybrid protein for production of recombinant bacteriorhodopsin in Escherichia coli.
    Author: Nekrasova OV, Wulfson AN, Tikhonov RV, Yakimov SA, Simonova TN, Tagvey AI, Dolgikh DA, Ostrovsky MA, Kirpichnikov MP.
    Journal: J Biotechnol; 2010 Jun; 147(3-4):145-50. PubMed ID: 20363267.
    Abstract:
    Unique properties of bacteriorhodopsin, namely, photochromism and high thermal stability, make this protein an attractive target for physico-chemical studies, as well as for various biotechnological applications. Using Mistic as a suitable carrier for insertion of recombinant membrane proteins into cytoplasmic membrane of Escherichia coli, we developed a system for overexpression of bacteriorhodopsin and worked out an efficient procedure for its purification and renaturation with the final yield of 120 mg/l of refolded protein, which is the highest value reported to date for bacteriorhodopsin produced in E. coli. Functional activity of recombinant bacteriorhodopsin was confirmed by spectroscopic and electrochemical assays.
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