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  • Title: Occurrence of ceramide digalactoside as the main glycosphingolipid in the marine sponge Halichondria japonica.
    Author: Hayashi A, Nishimura Y, Matsubara T.
    Journal: Biochim Biophys Acta; 1991 May 08; 1083(2):179-86. PubMed ID: 2036451.
    Abstract:
    The main glycosphingolipid of the sponge Halichondria japonica was isolated and its structure was determined to be ceramide digalactoside, Gal alpha 1----4Gal beta 1----1Cer, using FAB/MS, IR, 1H-NMR and chemical methods such as permethylation analysis and degradation techniques. The ceramide moiety was composed mainly of 4-hydroxy-iso-octadecasphinganine and 2-hydroxy-docosanoic acid. Interestingly, the branched long-chain bases were comprised of 70% total long-chain bases and 17.5% 4-hydroxy-anteiso-nonadecasphinganine. Ceramide monohexoside, consisting of galactosyl ceramide (81%) and glucosyl ceramide (19%), was also isolated as a minor component. The composition of the ceramide moiety was nearly the same as that of ceramide digalactoside.
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